Analysis of endophytic bacterial diversity in seven pear varieties based on high-throughput sequencing

Pyrus spp. has a long history of planting, which is rich in varieties, high in nutritional and medicinal value. At present, there are few studies on the endophytic bacterial diversity of pear, which limits the development and utilization of pear endophytic bacterial resources. To explore the diversity of endophytic bacteria in pears and discover the potential microbial resources and functions, high-throughput sequencing was used to determine the V4-V5 region of 16S rRNA gene of endophytic bacterial community in seven pear varieties, and bioinformatics analysis was carried out. Among the seven varieties, 30 001−42 255 effective sequences were obtained, and 33−99 operational taxonomic units (OTUs) were clustered. These sequences were divided into 17 families, 28 classes, 52 orders, 85 families and 120 genera. In addition, the Shannon index, Simpson index and Chao index of endophytic bacteria in seven pear varieties were 0.481−1.154, 0.452−0.730 and 42.333−103.875, respectively. The data indicated that the endophytic bacteria of the seven tested varieties were rich, Fengshui pear and Crystal pear had the most abundant endophytic bacteria. In different taxonomic categories, the main endophytic bacteria types of different pear varieties were the same at the level of phylum, class, order, family and genus, but the practical proportion is different. The diversities of endophytic bacteria in the seven pear varieties were ralatively rich, and there were some differences in the bacterial community structure of different pear varieties. The reference for further revealing the interaction between pear and endophytic bacteria was provided.