Establishment of a method for determination of three saponins composition from Panax notoginseng extract in rats and pharmacokinetic study

To verify the method of HPLC-MS/MS determination of notoginsenoside R₁, Ginsenoside Rg₁, Ginsenoside Rb₁ in plasma of SD rats, the pharmacokinetics of Panax notoginseng extract in SD rats was studied. SD rats intragastrically administered Panax notoginseng extract of 1500 mg/kg had their blood collected for the determination of plasma concentration by HPLC-MS /MS, after which pharmacokinetic parameters were calculated by DAS software. The established HPLC-MS/MS method has good specificity, and the correlation coefficients of the three consecutive batches of standard curves are all greater than 0.99 (weighted 1/X²). The linearity of each component in certain concentration ranges was good. The RSD day precision of three components in plasma were less than 10% and the accuracy ranged from 88% to 105%. The diurnal precision (RSD) was less than 15% and the accuracy was between 93% to 109%. The stability of freeze-thaw and room temperature was good. The matrix effect does not affect the measurement effect. The non-compartmental model was used to calculate the pharmacokinetic parameters of Rg₁, Rb₁ and R₁ in rats. The results showed that the average values of AUC_0-t, ρ_max, t_1/2, and MRT_0-t, Rb₁>>Rg₁>R₁; average T_max value of Rb₁>> R₁> Rg₁. The established HPLC-MS/MS method is suitable for the pharmacokinetic study of Rg₁, Rb₁ and R₁ in Panax notoginseng extract in rats. Rg₁ and R₁ have similar pharmacokinetic characteristics in rats, with rapid absorption and fast elimination; Rb₁ pharmacokinetic characteristics in rats are very different from Rg₁ and R₁, with slow absorption and slow elimination; Rb₁ exposure inplasma of rats take the absolute advantage.