Studies on secondary metabolites of an Actinomycete Actinorectispora metallic KC 198^T from Bayan Obo mining area

It is studies on the secondary metabolites of metalli KC 198^T from from Bayan Obo mining area, and their antioxidant activities. A. metalli KC 198^T was fermented by PDB liquid, and the fermentation products were extracted by ethyl acetate. The secondary metabolites were isolated by silica column chromatography, Sephadex LH-20 column chromatography and RP-18 reverse-phase column chromatography. Then, the structures of the compounds were elucidated on the basis of spectral characteristics (NMR, MS) and comparison with the data of literatures. Then, the antioxidant activity of these compounds was tested by DPPH. From the ethyl acetate extracts of A. metalli KC 198^T, seven compounds were isolated and identified as ( 1 ) 1,4-dimethoxy-2-hydroxynaphthalene-2-O-β-D-glucopyranoside, ( 2 ) 4-hydroxy-tetralone, ( 3 ) Cyclo -(L-Trp-L-Pro), ( 4 ) uracil, ( 5 ) thymine, ( 6 ) β-adenosine, ( 7 ) 3-(2,3-dihydroxypropyl) indole. The antioxidant activity of the isolated structures was measured by DPPH method, at the concentration of 50 μmol/mL, the above compounds did not show good antioxidant activity. Compound 1 was a new compound, and the other compounds were isolated from this bacterial type strain for the first time. This study laid a foundation for further excavated the metabolites of microbes in Bayan Obo mining area.