Study on the secondary metabolites from mangrove-derived endophytic fungus Talaromyces assiutensis sp.

In order to search for anti-fungal secondary metabolites from endophytic fungi of mangroves, a mangrove-derived endophytic fungus Talaromyces assiutensis sp. from the mangrove Ceriops tagal was studied. Fifteen secondary metabolites (1−15) including 5-acetamidopentanoic acid (1), methyl-4-acetamidbutyrate (2), pyrophen (3), (E)-6-hydroxy-5- (1-propenyl)-1,2-dihydropyrano3,2-bpyrrole-3,7-dione (4), N-acetyltyramine (5), fonsecinone A (6), aurasperone A (7), asperpyrone D (8), aurasperone F (9), isoherqueinone (10), ergosterdiacids B (11), kojic acid (12), L-1-methyl citrate (13), methyl p-hydroxyphenylacetate (14) and p-hydroxyphenylacetic acid (15) were isolated from the T. assiutensis sp. by using modern chromatographic separation technology. Their structures were elucidated of the basis of spectroscopic and chemical data, especially NMR and MS. Compounds 1 and 2 are new natural products, which ¹H/¹³C NMR data were reported for the first time. Compounds 1−15 were evaluated their anti-fungal activities, in which compound 6−10 showed anti-fungal activities.