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GUO Chun-lei, WANG Tao, ZHU Wei, CHAI Li-hong, ZHANG Dong-hua, CUI Xiao-long, XU Li-hua, PENG Qian. The 16S rRNA of Thermus thermophiles from two high temperature hot springs of West YunnanJ. Journal of Yunnan University: Natural Sciences Edition, 2003, 25(5): 458-462.
Citation: GUO Chun-lei, WANG Tao, ZHU Wei, CHAI Li-hong, ZHANG Dong-hua, CUI Xiao-long, XU Li-hua, PENG Qian. The 16S rRNA of Thermus thermophiles from two high temperature hot springs of West YunnanJ. Journal of Yunnan University: Natural Sciences Edition, 2003, 25(5): 458-462.

The 16S rRNA of Thermus thermophiles from two high temperature hot springs of West Yunnan

  • Based on culture dependent method and culture independent method,several Thermus 16S rDNA sequences were amplified.The results of 16S rDNA phylogenetic analysis and 16S rRNA secondary stucture comparison showed that there are at least two novel Thermus species in the two hot springs and they constituted an independent cluster on the phylogenetic tree.The secondary structure of 16S rRNA helix 10 with novel evolution characters also showed differences with other Thermus species.
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