In vitro rapid propagation from young leaf of Sinephropteris delavayi(Franch.) Mickel
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Abstract
Young leaves from Sinephropteris delavayi (Franch.)Mickel were used for tissue culture and rapid propagation.This experiment showed that the most proper medium for inducing green globular bodies (GGB) was 1/2MS+6-BA 3.0mg/L+NAA 0.5mg/L(sucrose 3%),the medium for multiplying GGB was 1/2MS+6-BA 2.0mg/L+NAA+0.2mg/L(sucrose 2%), the medium for GGB to shoot was 1/2MS+NAA0.3mg/L(sucrose 2% and no hormone).1/2MS (sucrose 2% and no hormone)was good for rooting,the rooting rate was 100%.
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