Symbiotic seed germination of Cymbidium mastersii Griff.ex Lindl

In order to screen mycorrhiza fungus that can promote the seed germination and form the symbiosis with Cymbidium mastersii Griff.ex Lindl and scale produce high quality seedling,we first symbiosis cultivated seeds with different fungi in the medium of cortices symbiotic culture,then measured the biomass of seedlings,re-separation of fungus strain,studied the 3D hypha net by using the optical microscope and electron microscope,and determined the seed vitality with TTC method.The results showed that strain CLB111 and MLX102 that were separated from roots of Cymbidium goeringii Rchb.f.and C.Sinense Willd can promote the seed germination of Cymbidium mastersii.The differences of germination rates between treatments with strain symbiosises and control were significant.Re-separations from the culture medium confirmed the strains in symbiosis roots were the same as the inoculated strains.The optical microscope and electron microscope observation found that many fungus hypha infected into embryo cell and formed the 3D hypha net,and the embryo started differentiation.TTC measurement showed that the seed had high vitality.No strain was found by re-separation from culture medium,no hypha was observed and very low vitality under control treatment.Thus,It can be concluded that strain CLB111 and MLX102 can form the symbiosis and promote the seed germination of Cymbidium mastersii.The experiments found the fungus separated from adult orchids and the fungus that promote the seed germination were the same strains in Cymbidium mastersii.This phenomenon was different from the Gastrodia elata Blume.It was also confirmed that funguses that can form symbiosis with and promote the seed germination of Cymbidium mastersii were not absolutely specific.Under certain condition,different strains can significantly promote germination of one orchid species.It may need further investigation to verify the differences under different ecological conditions.