Separation,structural characterization and inducing proliferation activityof Friedelin-3,4-lacton from Calophyllum polyanthum

The methanol extract of the dried leaf of Calophyllum polyanthum was partitioned successively with petroleum ether and ethyl acetate.Petroleum ether fraction was gradient eluted over silica gel column using 9:1 petroleum ether- ethyl acetate as eluant to give a rod crystal and three known compounds.The rod crystal was characterized by IR,1H NMR,13C NMR,MS and X-ray single crystal diffraction.The results showed that the crystal was friedelin-3,4-lactone,and the molecular formula was C30H50O2. Its bioactivity was measured by MTT assay.The results proved that triterpenoid lactone has a promotion effect on the inducing proliferation of rat mesenchymal stem cells(rMSCs).