Study on sugars in Lithhocarpus polgstachrch Rehd and Houttuynia solid phase extraction and RP-HPLC

Determination of sugars in Lithhocarpus polgstachrch Rehd and Houttuynia with HPLC and include the contents of water soluble sugars and the component of polysaccharides.The water soluble sugars chromatography were extracted with water by ultrasonic oscillation and by solid phase extraction with Sep-pak C18 cartridge.Sugars were separated on a Waters high performance carbohydrate column as solid phase,with V (acetonitrile):V (water)=70:30 as mobile phase,and detected with evaporative light-scatter detector.The method was applied to the determination of water soluble monosaccharides and polysaccharides at the same time.