陈金全, 王风平. 珠江口海岸带沉积物甲烷相关古菌群落结构及多样性研究[J]. 云南大学学报(自然科学版), 2013, 35(2): 240-245. doi: 10.7540/j.ynu.20120172
引用本文: 陈金全, 王风平. 珠江口海岸带沉积物甲烷相关古菌群落结构及多样性研究[J]. 云南大学学报(自然科学版), 2013, 35(2): 240-245. doi: 10.7540/j.ynu.20120172
CHEN Jinquan, WANG Fengping. The community structure and diversity of methanogen-related organisms in Pearl River Estuary sediment[J]. Journal of Yunnan University: Natural Sciences Edition, 2013, 35(2): 240-245. DOI: 10.7540/j.ynu.20120172
Citation: CHEN Jinquan, WANG Fengping. The community structure and diversity of methanogen-related organisms in Pearl River Estuary sediment[J]. Journal of Yunnan University: Natural Sciences Edition, 2013, 35(2): 240-245. DOI: 10.7540/j.ynu.20120172

珠江口海岸带沉积物甲烷相关古菌群落结构及多样性研究

The community structure and diversity of methanogen-related organisms in Pearl River Estuary sediment

  • 摘要: 用构建克隆文库的方法对珠江口沉积物中4个层位的甲烷相关古菌群落多样性特征进行研究.结果表明甲烷相关古菌群落结构主要可以分为3类,分别为Methanosaeta,Methanomicrobiales和Methanosarcinales/ANME,且其组成随沉积物深度的改变发生规律变化.Methanosaeta和Methanomicrobiales是属于乙酸和H2/CO2利用型甲烷产生菌,在克隆文库中的含量随沉积物深度的增加而减少;Methanosarcinales/ANME主要由甲烷氧化菌和甲基利用型甲烷产生菌组成,在克隆文库中的含量随沉积物深度的增加而增加.甲烷相关古菌群落结构的变化规律说明,在SMTZ区域及以上,甲烷产生以H2/CO2和乙酸型为主;在SMTZ区域以下,以甲基利用型为主;在SMTZ区域,主要是甲烷氧化过程,由古菌类群ANME-2a负责.

     

    Abstract: Based on clone and sequencing of methanogen-related 16S rRNA genes,the diversity and composition of methanogen-related archaeal communities in Pearl River Estuary sediment were investigated.The phylogenetic results suggested the methanogen-related archaeal communities in the sediment were mainly composed of three types of groups,Methanosaeta,Methanomicrobiales and Methanosarcinales/ANME respectively.The variation of detected archaeal phylotypes by depth indicated that Methanosaeta and Methanomicrobiales were predominant groups above SMTZ and the content decreased by depth;Methanosarcinales/ANME was dominant group within or below SMTZ and the content increased by depth.Methanosaeta and Methanomicrobiales mainly belong to acetate- and H2/CO2-utilizing methanogen, which indicated methanogenesis above SMTZ mainly depend on acetate-and H2/CO2 as substrates. Methanosarcinales/ANME was responsible for methane oxidation and methyl group-utilizing methanogenesis,which suggested that methane oxidation and methyl group-utilizing methanogenesis were main metabolic processes within and below SMTZ. The subgroup ANME-2a also was identified as methane oxidizer in Pearl River sediment.

     

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