Abstract: This paper developed a method for measuring phenolic acids in root exudates of rice by solid-phase extraction (SPE)-high performance liquid chromatography (HPLC).Phenolic acids were extracted from culture solutions using cleanert PEP (500mg/6mL) column.Chromatographic separation was performed with ZORBAX SB-C18 column（4.6mm150mm,5m）by using the mobile phase gradient elution of methanol-0.1% phosphoric acid mixture,the flow rate is 1.6mL/min,the column temperature is 25℃ and the detection wavelength is 280nm.Under the optimum conditions,10 phenolic acids (3,4-dihydroxybenzoic acid,phydroxy-benzoic acid,vanillic acid,syringic acid,vanillin acid,ferulic acid,coumarin,benzoic acid,salicylic acid,cinnamic acid) were separated and determined.The developed method was successfully used to measure and analyze phenolic acids in the root exudates of rice.The average recoveries were 94.6%105.8%.This method is sensitive,rapid,accurate and with good repeatability.It is suitable for the determination of phenolic acids in the root exudates of rice.