Abstract: The chemical constituents of Pittosporum podocarpum Gagnep were studied.Seven of compounds were isolated from this plant by column chromatogramphies of silica gel.Their structures were identified by spectral data analysis.All these compounds were isolated for the first time from this plant.These are:ethyl caffeate(1);scopoletin(2);uvaol(3);3,4,5-trimethoxyphenol-1-O--D- glucopyranoside(4);3,4,5 -trimethoxyphenol-1-O--D-xylopyranosyl -(12)--D-glucopyranoside(5);syringaresinol- 4,4-di-O--D-glucoside(6);sucrose(7).Cytotoxicity screening of extracts and the compounds from Pittosporum podocarpum Gagnep was carried out by K562 cell.Three compounds(1,2,3 )possess cytotoxic activities and the IC50 are 4.0,12.5,13.5 g/mL for K562,respectively.