Abstract: From 600 different leaves of 4 cultivars of Cannabis sativa L.,376 endophytic fungi strains were isolated,of which 71 strains were from cultivar of Yunma No.3,107 strains were from Yunma No.4,95 strains were from Yunma No.5 and 103 strains were from Yunma No.6.The endophytic fungi were identified by both morphological and molecular method.The results indicated that endophytic fungi from industrial hemp could be divided into 20 genera,and 6 of them were found in all cultivars.Colletotrichum was the dominant genus,and the dominance index was 50% higher than all the other detected hemp cultivars.Isolation rate of endophytic fungi from hemp tissues ranged from 0.47 to 0.71,with 0.63 as an average.The study implied that the endophytic fungi existing in industrial hemp showed relatively low diversity and host specificity.