Abstract: Alkaloids from tea have many unique physiological activities,mainly containing theobromine theophylline and caffeine,there are a small number of pyrimidine alkaloids as well.A new reversed-phase-high performance liquid chromatographic procedure for the determination of alkaloids was described.The method has been used for separation and determination of the theobromine,theophylline and caffeine in tea and tea drinking.The results showed the method,which is simple,rapid,sensitive and of wide linear range,could be used for the determination of alkaloids in tea and be assessed the quality of ancient teas and its health function.