Abstract: The area of barley planting in Yunnan (perennial 220 000230 000 hectares) ranks the first in China.To explain the change of functional ingredients in barley grain and barley grass by different cut seedling periods and the correlation between the functional ingredients in barley grain and barley grass,tests of 12 barley lines from six provinces of Southern barley region were conducted in different cut seedlings period as stage Ⅰ(cut seedlings at the stages of three mature leaves and one new leaf ),stage Ⅱ(five mature leaf and one new leaf) and no cut seedlings (CK),which provided theoretical basis for screening out the dual functional barley varieties available both in producing grains and providing barley grass in Yunnan.The main results were as follow:① Total flavones in barley grains ranked as CKⅡ Ⅰ.Resistant starch in barley grains ranked as ⅠCKⅡ.There was no significant difference in the change of -aminobutyric and alkaloid,CKⅠⅡ was found in -aminobutyric;while CKⅠⅡ in alkaloid.It was the best way to cut seedlings at the stage I when the highest functional ingredients of barley grains were obtained.② Alkaloid and total flavones in barley grass ranked as Ⅱ＞I.There was no significant difference in -aminobutyric change in barley grass,and Ⅱ ＜Ⅰ.It was suitable to cut seedlings at the stage Ⅱ to have the highest functional Ingredients in barley grass.③ In different cut seedlings stages,functional ingredients of barley gains and grass showed difference among springness varieties,semi- winterness and winterness varieties;between poly-rowed barley and two-rowed barley.④ Correlation analysis showed that -aminobutyric acid between barley gains and barley grass was positively related,so was the total flavones in barley grass among different varieties,and -aminobutyric acid and total flavones of barley gains.There was positive correlation between yield trait and functional ingredients.⑤ Two dual -functional barley varieties:Supi3 and 2008ping22 were screened out.