Abstract: To discover new bioactive Sodium-dependent glucose cotransporter 2(SGLT2) inhibitors from the traditional Chinese medicine:Kushen(roots of Sophora flavescens),the bioassay-guided purification of an active methanolic fraction was performed.Seven known flavonoid glycosides were isolated and determined.Their structures were elucidated as formononetin 7-O- -D-xylopyranosyl-(16)- -D-glucopyranoside (1),formononetin 7-O--D-apiofuranosyl-(16)--D-glucopyranoside (2),trifolirhizin (3),(-)-maackiain 3-O--D-galactopyranoside(4),trifolirhizin 6-monoacetate (5),rothindin (6) and ononin (7).All compounds were isolated from this plant for the first time except compound 3.All seven compounds exhibited inhibitory activity against SGLT2.Among them,compounds 35 with the maackiain skeleton showed the most potential inhibitory activity against SGLT2 with the IC50 value ranging between 2.61 and 4.47 mol/L.