Abstract:
The aim of this study is to explore the cytotoxicity of CaCO3 particles on the human hepatocyte HL-7702 in vitro.HL-7702 cells have been treated with CaCO3 at the final concentrations of 5,10,20,40,60,80,100 μg/mL and saline has been used as control.CCK-8 assay which has been conducted to detect the effects on the survival rate of the cells exposed to CaCO3 for 24 h.The concentrations of reactive oxygen species (ROS) of HL-7702 cells have been determined.The results show that CaCO3 particles have no cytotoxicity and can induce the low levels of intracellular reactive oxygen species (ROS).These indicate that calcium carbonates particles are nontoxic in vitro cellular compatibility with their toxicity reaction ranking level 0 or 1.