胡月新, 刘建军, 黄芩, 韦焘, 林意菊. 不同剂量顺铂对体外培养食管癌细胞敏感性研究[J]. 云南大学学报(自然科学版), 2013, 35(S2): 390. doi: 10.7540/j.ynu.20130802
引用本文: 胡月新, 刘建军, 黄芩, 韦焘, 林意菊. 不同剂量顺铂对体外培养食管癌细胞敏感性研究[J]. 云南大学学报(自然科学版), 2013, 35(S2): 390. doi: 10.7540/j.ynu.20130802
Research on sensitivity of esophageal cancer cells developed in vitro using different doses of cisplatin[J]. Journal of Yunnan University: Natural Sciences Edition, 2013, 35(S2): 390. DOI: 10.7540/j.ynu.20130802
Citation: Research on sensitivity of esophageal cancer cells developed in vitro using different doses of cisplatin[J]. Journal of Yunnan University: Natural Sciences Edition, 2013, 35(S2): 390. DOI: 10.7540/j.ynu.20130802

不同剂量顺铂对体外培养食管癌细胞敏感性研究

Research on sensitivity of esophageal cancer cells developed in vitro using different doses of cisplatin

  • 摘要: 为探讨不同剂量的顺铂对体外培养食管癌细胞敏感性的影响,以高、中、低3种剂量的顺铂对体外培养的人食管癌细胞Eca-109进行处理,对照组以不同剂量顺铂处理人肺腺癌细胞A549,两组均采用MTT法测定药物对癌细胞的抑制作用.结果显示高、中、低剂量顺铂对人食管癌细胞Eca-109均有抑制作用,抑制率分别为(73.16±1.35)%,(39.76±1.04)%,(18.75±0.62)%,药物浓度越高抑制作用越强.作者认为顺铂浓度越高对食管癌细胞的增殖抑制作用越强,敏感度越高,可供临床治疗参考应用.

     

    Abstract: To discuss the effect of esophageal cancer cells,we developed them in vitro using different doses of cisplatin.Eca-109 esophageal carcinoma cells were processed in three experimental groups with high,medium and low dose of cisplatin,respectively,and A549 lung adenocarcinoma cells were processed using the same method in three control groups.Methylthiazol tetrazolium (MTT) method was used to determine the inhibitory effects.The results showed that different doses of cisplatin can inhibit the Eca-109 esophageal carcinoma cells.The inhibition rates were (73.16±1.35)%,(39.76±1.04)%,and (18.75±0.62)%,respectively,while the higher the dose,the stronger the inhibitory effect.So,cisplatin can inhibit the growth of esophageal carcinoma cells,and the higher the dose,the stronger inhibitory effect.

     

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