树鼩VEGF全长编码序列的克隆及分子特征分析

Cloning of full length coding sequence and prediction of the molecular characteristics of VEGF of tree shrews

摘要: 树鼩作为研究人类疾病的良好动物模型,近年来引起广泛关注.研究采用RT-PCR技术,从树鼩的肺组织中克隆到血管内皮生长因子（vascular endothelial growth factor,VEGF）的编码序列,并对其三级结构进行了预测.结果显示该基因开放阅读框全长645 bp,编码214个氨基酸.序列比对及三级结构预测显示,树鼩VEGF与人的VEGF有较高的相似性，预示树鼩可能会是研究人类血管相关疾病的良好模型.

Abstract: As a good animal model for the study of human diseases,tree shrew has drawn extensive attention in recent years.In this study,the coding sequence of vascular endothelial growth factor (VEGF) was cloned from lung tissue of a tree shrew using RT-PCR technology,and its three-dimensional structure was predicted.The results showed that the open reading frame of VEGF is 645 bp,encoding 214 amino acids.Sequence alignment and three-dimensional structure prediction showed that tree shrew VEGF has high similarity to human VEGF.It suggested that tree shrew might be a good model for the study of human vascular diseases.