三七茎叶中γ-氨基丁酸提取工艺研究

Optimization of extraction technique of GABA from the stems and leaves of Panax notoginseng

摘要: 探索三七茎叶中γ-氨基丁酸提取工艺.考察提取的溶剂、时间、次数、温度及物料比5个单因素影响,采用正交实验分析方法确定最佳工艺条件.其结果表明三七茎叶中γ-氨基丁酸的最佳提取工艺为:提取溶剂为水,提取时间3 h,提取次数3次,提取温度60 ℃,提取物料比1 g∶10 mL,在此条件下γ-氨基丁酸的提取率为0.46%.

Abstract: In this paper,the optimum conditions for the extraction of GABA from the stems and leaves of Panax notoginseng were determined.On the basis of single-factor experiments(extraction solvent,time,times,temperature,material ratio),the optimum condition for the extraction of GABA was determined by orthogonal experiment.The optimum extraction was based on three times for 3 h at 60 ℃ with water,and the material ratio was 1 g∶10 mL,and the content of GABA from the stems and leaves of Panax notoginseng was 0.46%,and these will provide the technical support for comprehensive development and utilization of the aerial part of Panax notoginseng.