Abstract: Three rounds of selection was carried to get two rows (each row has 8 positive clones) which could bind specifically to anti-HPV-58 E7 monoclonal antibody 4C4a and 6C7a through the lab platform by performing phage random 12-mer peptide library screening.The positive clones of the peptide sequences represent mimotopes which mimic the epitope of the target protein HPV-58 E7.After bioinformatic analysis of the positive peptides and the target protein sequence,a linear sequence alignment and analysis were done to make a comparison between them and a prediction for the conformational epitope was made by EpiSearch Server and Pepitope Server,which is very helpful in finding the conformational epitope and predicting the clusters,as well as providing support to early diagnosis and development of the molecular medicine.