陈兴, 王文元, 汪显国, 党立志, 赵英良, 颜克亮, 凌军, 段焰青. 烟叶纤维素降解菌的筛选、鉴定及其产酶条件优化[J]. 云南大学学报(自然科学版), 2015, 37(2): 323-328. doi: 10.7540/j.ynu.20140343
引用本文: 陈兴, 王文元, 汪显国, 党立志, 赵英良, 颜克亮, 凌军, 段焰青. 烟叶纤维素降解菌的筛选、鉴定及其产酶条件优化[J]. 云南大学学报(自然科学版), 2015, 37(2): 323-328. doi: 10.7540/j.ynu.20140343
shu
CHEN Xing, WANG Wen-yuan, WANG Xian-guo, DANG Li-zhi, ZHAO Ying-liang, YAN Ke-liang, LING Jun, DUAN Yan-qing. Screening,identification of cellulose-decomposing strain from aging flue-cured tobacco leaves and optimization of its fermentation condition[J]. Journal of Yunnan University: Natural Sciences Edition, 2015, 37(2): 323-328. DOI: 10.7540/j.ynu.20140343
Citation: CHEN Xing, WANG Wen-yuan, WANG Xian-guo, DANG Li-zhi, ZHAO Ying-liang, YAN Ke-liang, LING Jun, DUAN Yan-qing. Screening,identification of cellulose-decomposing strain from aging flue-cured tobacco leaves and optimization of its fermentation condition[J]. Journal of Yunnan University: Natural Sciences Edition, 2015, 37(2): 323-328. DOI: 10.7540/j.ynu.20140343
shu

烟叶纤维素降解菌的筛选、鉴定及其产酶条件优化

Screening,identification of cellulose-decomposing strain from aging flue-cured tobacco leaves and optimization of its fermentation condition

    摘要
  • 摘要: 利用筛选培养基从陈化烟叶中分离到纤维素(CMC)酶活性较高的菌株CE1,对其进行鉴定后表明CE1为绿色木霉 (Trichoderma viride).对其产酶条件进行优化,表明CE1的最佳产酶初始pH为6.5,最适产酶温度28 ℃,培养至第5天时CMC酶活达到最大值.利用其粗酶液对烟丝进行了处理,表明随着处理浓度的提高,烟丝纤维素的降解率也随之升高,烟丝总糖的含量也有所提升.

     

    Abstract: A cellulose-decomposing strain CE1 with high enzyme activity was isolated from aging flue-cured tobacco leaves by selection medium and classified as Trichoderma viride.The optimum pH value of the fermentation of CE1 was 6.5,the optimum temperature was 28 ℃,the carbomethyl cellulase(CMC ase) got the highest activity at the 5th day of culture.Crude enzyme of CE1 was used directly for tobacco processing,the results showed that the degradation rate and total sugar of tobacco increased by increasing the concentration of the enzyme.

     

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