Abstract: :Fourteen of imprinted polymers with stereoselectivity to L-tryptophan were prepared with DL-Trp as template,β-cyclodextrin(β- CD) and its derivatives as functional monomers to build a chiral environment.The imprinting system was optimized systematically by varying the imprinting components and polymerization process.The optimum imprinting polymer (MIP14) was characterized in structure,binding characteristics and chiral resolution ability.The obtained data revealed a mesoporous material with two classes of binding sites and pesdo-second dynamics adsorption model for L-Trp.MIP14 shows a good chiral resolvability to racemic tryptophan.Even in the complex system composed by racemic tryptophan,tyrosine and phenylalanine,MIP14 can recognize L-Trp superior to D-Trp with a resolution factor of 1.477.The results demonstrate the feasibility to prepare chiral molecularly imprinted materials with racemic molecules.The results demonstrated the route that imprinting racemic mixtures with β-CD is more easy and practical compared than the traditional imprinting methods.