Abstract: In order to shorten the time of tag identification and improve the efficiency of system identification,based on the queuing theory,a tag identification process optimization algorithm in RFID system with multi-readers is proposed,the analysis model is built by combining the optimal control theory.According to the discrete queuing mechanism of queuing theory,the study suggested that a variable order identification model for multi-readers was an efficient approach to dealing with the long queues compared with single reader mode.The algorithm divided tags into few groups through random assignment,and novelly introduced the first reader identification order to tags.By considering the transformation time among readers and the satisfaction of tags,along with the estimation of tag numbers in each group,the optimization orders come with different first reader situations can be obtained.The simulation results shows that the optimal orders generated by established model reflect the advantages of low satisfaction level and high speed of identification,and avoid the long waiting time due to large numbers of tags gathered in a group,thus it verifies the reliability of the algorithm.