Abstract: DNA extraction is key to the screening and identification process of Arabidopsis mutants.Based on the characteristics of Arabidopsis mutants identification and DNA extraction, a simple, fast and economical DNA extraction method was worked out and applied into the batch screening and identification of mutants through modifying the traditional SDS extraction method.By using this method, DNA templates for PCR testing could be prepared quickly at room temperature from about 10mg leaves, a small amount of reagent, and easy operation steps of grinding, centrifugation, sedimentation, and drying.A single DNA sample can be obtained in only about 15 minutes and 48 DNA samples are able to be extracted in an hour by the method.In this study, DNA samples of a batch of Arabidopsis mutants to be identified were prepared by applying the method, and then was detected by three primer PCR methods.The results showed that the concerntration of DNA samples was high, the success rate of DNA amplification was up to 100% and the target bands were clear and ideal, which indicates that the method is suitable and efficient, and can be widely used for mutant library constructions in the laboratory.