MCP-1和CCR2在SE初期小鼠脑海马中的表达变化

Change of expression of MCP-1 and CCR2 in the mouse hippocampusat the early stage of pilocarpine-induced epilepticus

摘要: 为了探讨趋化因子MCP-1及其受体CCR2是否参与了SE初期的病理过程,应用SE的匹罗卡品模型,检测SE 30min、SE 1h、SE 2h和SE 1d时,MCP-1及其受体CCR2的mRNA在小鼠海马的表达情况.结果显示,MCP-1及其受体CCR2在小鼠海马组成型表达.与对照组相比,SE 1h时,MCP-1在海马CA1区显著下调(P 0.01);SE 1d时,CCR2在CA3区显著下调(P 0.05).结果说明,MCP-1及其受体CCR2在成年小鼠海马的活动中具有重要的生理功能;MCP-1/CCR2的下调与SE初期病理过程相关,可能由于下调导致了它们的神经保护作用减弱.

Abstract: To investigate if chemokine MCP-1 and its receptor CCR2 participate pathogenesis at the early stage of epilepsy, expression of mRNA of the two genes in mouse hippocampus under SE 30min, SE 1h, SE 2h and SE 1d was investigated using pilocarpine model of SE. The results showed that MCP-1 and CCR2 were expressed in normal hippocampus of mouse constitutively.Compared to control group, MCP-1 was significantly downregulated in CA1 of hippocampus at SE 1h (P0.01)and CCR2 was significantly downregulated in CA3 at SE 1d (P0.05), which suggests that MCP-1 and CCR2 might play important roles in hippocampus.The downregulation of MCP-1 and CCR2 in the hippocampal neurones at the early stage of SE may weaken the neuroprotective mechanisms.