陈良华, 徐睿, 张健, 杨万勤, 胡相伟, 张明锦, 高顺. 螯合剂对香樟生理特征和镉积累效率的影响[J]. 云南大学学报(自然科学版), 2016, 38(1): 150-161. doi: 10.7540/j.ynu.20150523
引用本文: 陈良华, 徐睿, 张健, 杨万勤, 胡相伟, 张明锦, 高顺. 螯合剂对香樟生理特征和镉积累效率的影响[J]. 云南大学学报(自然科学版), 2016, 38(1): 150-161. doi: 10.7540/j.ynu.20150523
CHEN Liang-hua, XU Rui, ZHANG Jian, YANG Wan-qin, HU Xiang-wei, ZHANG Ming-jin, GAO Shun. The effects of chelator on physiological traits and Cd accumulation efficiency of Cinnamomum camphora[J]. Journal of Yunnan University: Natural Sciences Edition, 2016, 38(1): 150-161. DOI: 10.7540/j.ynu.20150523
Citation: CHEN Liang-hua, XU Rui, ZHANG Jian, YANG Wan-qin, HU Xiang-wei, ZHANG Ming-jin, GAO Shun. The effects of chelator on physiological traits and Cd accumulation efficiency of Cinnamomum camphora[J]. Journal of Yunnan University: Natural Sciences Edition, 2016, 38(1): 150-161. DOI: 10.7540/j.ynu.20150523

螯合剂对香樟生理特征和镉积累效率的影响

The effects of chelator on physiological traits and Cd accumulation efficiency of Cinnamomum camphora

  • 摘要: 植物修复效率常常受限于污染土壤中较低的重金属生物有效性,添加螯合剂活化土壤中的重金属以提升植物修复效率是一种重要的技术措施.采用盆栽实验研究了EDTA(0.25mmol·kg-1)和柠檬酸(0.2mmol·kg-1)对Cd污染条件下(10mg·kg-1)香樟(Cinnamomum camphora)幼苗生物量积累、生理特征、养分含量以及Cd积累量的效应.结果表明,在Cd污染条件下,单独添加EDTA、柠檬酸以及复合添加2种螯合剂一定程度增加了香樟幼苗的细根生物量,但对其它器官生物量、总生物量以及根冠比没有显著影响;添加以上螯合剂增强了叶片超氧化物歧化酶(SOD)的活性,降低了过氧化物酶(POD)的活性,但并未显著影响丙二醛(MDA)的含量;单独添加EDTA增加了细根的N含量(质量分数约14%)以及叶片的P含量(质量分数约15%),添加柠檬酸及复合添加2种螯合剂降低了香樟细根的N含量(均降低约20%)以及茎的P含量(分别降低约41%和36%);单独添加柠檬酸并没有显著促进各器官Cd的吸收和积累,但单独添加EDTA和复合添加2种螯合剂显著增加了香樟各器官Cd的含量和积累量,与单独添加2种螯合剂相比,复合添加2种螯合剂对Cd的积累表现出协同促进作用.这些结果表明,复合添加2种螯合剂的条件下,虽然香樟幼苗Cd的吸收和积累效率最高,但N、P等养分却受到更显著的负面影响,可能会对香樟的后续生长和Cd积累效率产生负面效应,因此认为单独施加EDTA能够提升Cd抽提效率,并且不会对香樟的生理特征产生负面影响,是比较适宜的方法.

     

    Abstract: Phytoremediation efficiency is usually restricted by the low bioavailability of heavy metals in the polluted soil. Application of chelator is an important technology for the improvement of phytoremediation efficiency,which could activate the heavy metals in the soil. In our study,a pot-cultured experiment was conducted to detect the effect of EDTA (0.25mmol·kg-1 soil) and citric acid (0.2mmol·kg-1 soil) on the morphological growth,physiological traits,nutrient content,and Cd accumulation in Cinnamomum camphora when the soil was polluted by Cd (10mg·kg-1 soil).The results included that:① Under Cd-polluted condition,the addition of EDTA,citric acid and both all increased the biomass of fine roots to a certain degree,but did not significantly affect the biomass of other organs,total biomass and the ratio of root to shoot.② The application of both chelator increased the activity of superoxide dismutase (SOD) significantly,but decreased the activity of peroxidase (POD),the content of malonaldehyde (MDA) was not significantly affected.③ EDTA alone increased N content in fine roots by 14 and P content in leaves by 15%. In contrast,the application of citric acid alone and a combining application of EDTA and citric acid decreased N content in fine roots by 20%,and P content in stems by 41% and 36%,respectively.④ Citric acid did not affect Cd content and its accumulation amount significantly in all organs.However,EDTA alone and the combination increased Cd content and accumulation amount significantly in all organs. Compared to a single application of chelator,there was a synergetic increase in Cd accumulation under interaction treatment. Such results indicated that although the maximum accumulation of Cd in plants achieved under the combined application of chelator,both N and P status were negatively affected when compared to Cd stress alone,which might induce negative effects on growth and Cd accumulation efficiency in the future. Therefore,it is recommended that the application of EDTA is a proper assistant method for phytoremediation.

     

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