Abstract:
The solvent extraction technology of flavonoids in Indigofera atachyoides Lindl was optimized by orthogonal experiments.The experimental results showed that the order of different factors affecting extraction rate was content of ethanol,temperature,time,rate of solid/liquid.The optimal extraction conditions were 60% ethanol content,90℃ temperature,1∶30 rate of solid/liquid,2h extraction time,three times.In vitro antioxidative actity of the extract was evaluated by two systems,viz.DPPH radical scavenging activity and pyrogallol autoxidation methods.The results indicated flavonoids in Indigofera atachyoides Lindl have strong antioidative activity in vitro.