Abstract:
By comparing static-adsorption and desorption rate of HP-20,D101,X-5,LX-68,AB-8,XDA-6,XDA-8 and D201 macroporous resins,XDA-6 was considered to be the optimum resin to purify the crude flavonoids of raspberry dry fruits.By combining the static and dynamic experiments of resin adsorption-desorption,the optimum purification process was as follow:Taking crude flavonoids solution as sample,loading velocity at 6BV/h (1 column volume as 1BV),60 % alcohol as elution agent with 4BV/h elution velocity,and 5BV eluent volume.The purified flavonoids content by the process was 35.8 %,which was 1.21 times more than that of the unpurified flavonoids solution.When dry mass concentration was 0.5mg/mL,the antioxidant activity of total flavonoids to DPPH increased from 62.51 % to 70.36 % after purification.The antibacterial effect of purified total flavonoids on Escherichia coli,Staphylococcus aureus,Cotton fusarium Wilt vasinfectum and Fusarium graminearum was better than unpurified flavonoids.