Abstract: Tamarindus indica Linn. is an important economic tree species in dry hot valley areas which has multiple values. Illumina high-throughput sequencing technology and MISA software were used to develop whole genome SSR sequences of the tamarind.7 types,9447 SSR sequences were obtained and most of them were single nucleotide repeat type.The content of A/T base was significantly higher than that of G/C base in different types;and the length variation of microsatellite of single nucleotide repeat type was the highest.The length of each repeat type microsatellite was negatively correlated with the frequency of microsatellites.Results obtained from microsatellite sequences and the analysis comprehensively reflect the characteristics of T. indica genomic microsatellites and the potential functions,which will contribute to a series of subsequent genetic studies of this species.