祁超, 寸海春, 何鹏飞, 李兴玉, 吴毅歆, 何鹏搏, 何月秋. 生防菌YN201490在黄瓜植株体内的定殖能力及防病机制的初步研究[J]. 云南大学学报(自然科学版), 2019, 41(1): 172-180. doi: 10.7540/j.ynu.20170297
引用本文: 祁超, 寸海春, 何鹏飞, 李兴玉, 吴毅歆, 何鹏搏, 何月秋. 生防菌YN201490在黄瓜植株体内的定殖能力及防病机制的初步研究[J]. 云南大学学报(自然科学版), 2019, 41(1): 172-180. doi: 10.7540/j.ynu.20170297
QI Chao, CUN Hai-chun, HE Peng-fei, LI Xing-yu, WU Yi-xin, HE Peng-bo, HE Yue-qiu. Colonization ability in cucumber plant and biocontrol mechanism of Bacillus subtilis YN201490[J]. Journal of Yunnan University: Natural Sciences Edition, 2019, 41(1): 172-180. DOI: 10.7540/j.ynu.20170297
Citation: QI Chao, CUN Hai-chun, HE Peng-fei, LI Xing-yu, WU Yi-xin, HE Peng-bo, HE Yue-qiu. Colonization ability in cucumber plant and biocontrol mechanism of Bacillus subtilis YN201490[J]. Journal of Yunnan University: Natural Sciences Edition, 2019, 41(1): 172-180. DOI: 10.7540/j.ynu.20170297

生防菌YN201490在黄瓜植株体内的定殖能力及防病机制的初步研究

Colonization ability in cucumber plant and biocontrol mechanism of Bacillus subtilis YN201490

  • 摘要: 为研究枯草芽孢杆菌YN201490在黄瓜体内的定殖能力及防病机制,采用平板对峙试验的方法测试该菌株对不同病原菌的抑制作用,利用灌根的方法研究其在黄瓜体内的定殖能力,并通过抑制孢子萌发对其发酵液进行了初步分离与活性检测. 研究通过平板对峙试验证明了枯草芽孢杆菌YN201490对小麦雪腐病菌、番茄枯萎病菌、烟草赤星病菌、玉米拟轮枝镰孢菌、茶轮斑病菌和水稻叶鞘腐败病菌有很好的抑菌活性. 用自然转化法以GFP标记,获得了荧光标记菌株YN90-P43GFPmut3a. 通过灌根法,证明该菌株能在黄瓜植株体内定殖,其数量分布为根>叶>茎. YN201490发酵液的正丁醇萃取物能裂解小麦雪腐病菌孢子,使胞质外流,抑制91.72%孢子萌发,对黄瓜白粉病的预防效果达66.26%,并能诱导黄瓜产生明显抗性. 研究结果表明枯草芽孢杆菌YN201490对多种病原真菌具有明显的抑制作用,并能够在黄瓜体内稳定定殖,具有作为生物菌剂开发利用的潜能.

     

    Abstract: To understand the colonization ability in cucumber plant and biocontrol mechanism of Bacillus subtilis YN201490, plate confrontation was proceeded to test the inhibitory effect of the strain on different pathogens and the method of drenching root was used to study the colonization ability in cucumber, and the isolation and activity of the fermentation suspension was tested by inhibiting spore germination. Antagonistic activity of YN201490 against Typhula incarnata, Fusarium oxysporum f. sp. lycopersici, Alternaria alternata, F. verticillioides, Pestalotiopsis theae and Sarocladium oryzae was confirmed by the confrontation test and a GFP-labeled strain respectively, YN90-P43GFPmut3a was obtained to analyze the colonization ability. The results showed that strain could be colonize in cucumber plant with the colony numbers in root > leaf > stem based on drenching root experiments. The n-butanol extract from fermentation suspension could split the spores of T. incarnata to make the cytoplasm exudate and inhibit 91.72% of spores’ germination. When it was sprayed on cucumber it also prevented 66.26% of cucumber powdery mildew and induced significant disease resistance. It is concluded that YN201490 has an obvious inhibitory effect on many pathogenic fungi and colonization ability in cucumber, which has the potential as a biological agent.

     

/

返回文章
返回