Abstract: In this study,firstly,the leaves of Australian raspberry were used as materials,and the contents of total flavonoids,total SOD activity and total protein of leaves were investigated by the ultrasonic assisted ethanol extraction process.Based on the single factor experimental results of ethanol concentration,liquid ratio and ultrasonic time,Box Behnken experimental design and response surface methodology(RSM)were selected to further analyze and optimize the mixedextraction conditions.The finnal extraction process was 60﹪ethanol,solid-liquid ratio 1∶40(g / mL)and 30 minutes ultrasound. The optimum values were the total flavonoid content of 51.74 mg/g,SOD total activity of 5828.96 U and total protein content of 17.20 mg/g.Secondly,the optimum process was applied to 9 raspberry cultivars,including Torremi,Nova(with thorns),Nova(without thorns ),Heritage,Reveille,Mara Harding and Autumn Bliss(from the experimental field of Beijing Agricultural College,Beijing)and Australian raspberry(from the experimental field of North University of China,Shanxi),and the results showed that total flavonoids,total SOD activity and total protein contents of Black-red raspberry,Nova(with thorns)and Nova were higher than those of others,and those of Reveille and Heritage were almost the lowest.Similarly,total antioxidant activities of black raspberry and Nova(with thorns)were higher than those of Heritage and Reveille by DPPH analysis.Overall the results provided support for the introduction,selection and comprehensive utilization of raspberry.