Abstract: Microbe cement provides superior solutions for the protection of important buildings and precious stone relics,few independent intellectual property rights of the efficient calcium carbonate mineralization strain in China.In this study,856 strains of bacteria were isolated from soil and weathered products in Shilin Karsts of Yunnan,185 of them had urease activity,29 of them could induce the formation of calcium carbonate,6 of them could induce the formation of calcium carbonate efficiently.According to the physiological characteristics of the strain,SL313 was selected to optimize the medium composition by using response surface methodology.SL313 was identified as Staphylococcus xylosus,which has never been studied about the calcium carbonate deposition before.The results showed that the optimal condition was pH=6.00,urea 126mg/mL,calcium nitrate 344.4mg/mL,tryptone 25g/L,and the CaCO3 production reached 0.165g/mL,which was 17.9% higher than that under the initial culture conditions.Scanning electron microscopy and X-ray diffraction analysis was used to observe and study the crystal structure and chemical composition of the formed calcium carbonate.The results showed that SL313 could accelerate the formation of vaterite crystal.This study provides a large number of candidate strains for the development of microbial cement and new ideas for the utilization of microbial resources in Karsts.