Abstract: 1804 samples of C3F tobacco leaves including K326,Hongda and Yunyan87 were chosen from 12 tobacco-growing areas in Yunnan province.The chemical components contents among cultivars were analyzed applying multiple comparisons and the regional characteristics were discussed by cluster analysis.The results showed that:①There was significant difference of chemical components among cultivars.The sugar/nicotine ratio of three cultivars was slightly higher,and the chlorine content was slightly lower and the potassium/chlorine ratio was higher for Yunyan 87 cultivar.②The tobacco leaves sugar contents of K326 and Yunyan87 were lower and the total nitrogen,nicotine and the petroleum ether extract contents were higher in Zhaotong.However the sugar contents were higher and medium in other sample counties.Meanwhile the potassium/chlorine ratio of K326 and Yunyan87 were higher in Changning,Mojiang and Gejiu,Xinping,Lianghe respectively.③The reducing sugar and starch content of Hongda in Heqing and the total sugar content in Xinping were lower.The potassium/chlorine ratio of Hongda were higher in Lianghe and Ning'er.