Abstract: Based on the structure of cochinchinenin A,twenty conchinchinenin A derivatives were designed and synthesized via two steps reactions of acetophenone derivatives and 2,6-dimethoxybenzaldehyde.The structures of the obtained compounds were characterized by 1H NMR,13C NMR,IR and HRMS.And on this basis,with tramadol hydrochloride as positive control group,the writhing test the analgesic activity of the synthesized compounds was evaluated.The biological results demonstrated that compounds 4d1(8.33±1.45,13.00±4.16,8.67±3.71) and 5e(5.67±2.40,12.67±1.45,13.00±5.68) were commensurate with tramadol hydrochloride (6.67±1.20) in analgesic activity. The studies provide a reference for the further development of Dragon's blood in analgesic activity.