Abstract: Based on the data of land use status, land use change and yearbook of Yining City, the author explored the change of land use structure and its humanistic driving factors in the research area. The sequential variation and spatial differentiation of land use structure were analyzed by means of information entropy, equilibrium degree and dominance degree. From 2009 to 2015, the area of urban villages and industrial and mining land increased by 8 198.50 hm², while the grassland decreased by 7 777.74 hm². The change rate and dynamic degree were obvious. The time dimension of information entropy of land use structure is divided into ascending area, stable area and descending area, and the spatial dimension of regional difference is high entropy value, medium entropy value and low entropy value. The dynamic factors of land use structure change were studied by principal component analysis. The cumulative variance contribution rate of the three principal component factors was 93.415%. The results show that the change trend of urban villages, industrial and mining land and grassland is obvious during the study period, and the main controlling factors of spatial and sequential differentiation of land use structure information entropy are economic scale, radiation effect of central urban area and policy orientation, and population density, the proportion of tertiary industry to GDP, total retail sales of consumer goods, investment in state-owned fixed assets, and per capita consumption expenditure of rural residents are the most significant components.