Abstract: Skin precursor cells (SKPs) are multipotent stem cells derived from the dermis. SKPs are able to induce hair follicle regeneration. It is considered to be seed cells for the thereby of the skin injury. Their application is limited by the instability of their property during culture expansion. In this study, we employed epidermal cell conditioned medium to treat SKPs and found that the proliferation rate of SKPs was increased. In addition, the expression levels of SKPs signature genes Akp2, Sox2, Bmp4, Nestin1, PDGFa, Clstn2, Trps1 and Versican were up-regulated. In addition, the activity of alkaline phosphatase was enhanced significantly which reflect the hair follicle inducing ability of SKPs. Our study indicates that factors released by epidermal cells facilitate the stability of SKPs properties.