Abstract:
Microplitis bicoloratus bracovirus (MbBV) exist in the oviduct epithelial cells of braconidae, and it is crucial to the parasite of
Microplitis bicoloratus on
Spodoptera litura. Reactive oxygen species (ROS), which are produced by organelles such as mitochondria, are widely involved in various physiological and pathological processes. To study the role of ROS in the apoptosis of insect cells induced by MbBV, the correlation between ROS and apoptosis in the MbBV-infected cells was studied through in vivo and in vitro experiments. The results showed that ROS was upregulated in the larvae hemocytes of
Spodoptera litura after 6-day parasite,while the aopotosis of hemocytes increased. Similar results were shown in Spli221 cells infected by MbBV after 24 hours, implying that the upregulation of ROS is linked to MbBV infection in insect cells. Notably, when the formation of ROS was inhibited by the antioxidants NAC, MbBV-induced apoptosis was rescued. It is suggested that MbBV may induce apoptosis by promoting the production of ROS.