龙章德, 姜宇, 刘鸿, 李季刚, 孙建生, 胡志忠, 苏赞, 许春平. 不同西方许旺酵母接种量对复烤后烟叶增香提质的影响研究[J]. 云南大学学报(自然科学版), 2019, 41(4): 797-804. doi: 10.7540/j.ynu.20180712
引用本文: 龙章德, 姜宇, 刘鸿, 李季刚, 孙建生, 胡志忠, 苏赞, 许春平. 不同西方许旺酵母接种量对复烤后烟叶增香提质的影响研究[J]. 云南大学学报(自然科学版), 2019, 41(4): 797-804. doi: 10.7540/j.ynu.20180712
LONG Zhang-de, JIANG Yu, LIU Hong, LI Ji-gang, SUN Jian-sheng, HU Zhi-zhong, SU Zan, XU Chun-ping. Effect of inoculum amount of Schwanniomyces occidentalis on improving aroma and quality of redryed tobacco leaves[J]. Journal of Yunnan University: Natural Sciences Edition, 2019, 41(4): 797-804. DOI: 10.7540/j.ynu.20180712
Citation: LONG Zhang-de, JIANG Yu, LIU Hong, LI Ji-gang, SUN Jian-sheng, HU Zhi-zhong, SU Zan, XU Chun-ping. Effect of inoculum amount of Schwanniomyces occidentalis on improving aroma and quality of redryed tobacco leaves[J]. Journal of Yunnan University: Natural Sciences Edition, 2019, 41(4): 797-804. DOI: 10.7540/j.ynu.20180712

不同西方许旺酵母接种量对复烤后烟叶增香提质的影响研究

Effect of inoculum amount of Schwanniomyces occidentalis on improving aroma and quality of redryed tobacco leaves

  • 摘要: 为了使复烤烟叶增香提质,试验选用从烟叶表面筛选出的产香菌:西方许旺酵母(Schwanniomyces occidentalis)对兴义C3F复烤后烟叶进行固态发酵,并对烟叶的常规化学成分、香味物质和石油醚提取物含量进行检测,最后将发酵后的烟叶切丝后进行卷制成卷烟并进行评吸实验. 研究结果表明:①经菌种发酵后,接种量为6.9%和接种量为13.8%的烟叶糖碱比均升高,但钾氯比无显著性变化;②接种量为6.9%的处理中共检测出香味物质58种,与另外两组相比其中有50种香味物质含量较高,包括苯甲醇、苯乙醇、芳樟醇、香叶基香叶醇、螺岩兰草酮、苯乙醛、α-大马酮、巨豆三烯酮、橙花基丙酮、3-羟基-β-二氢大马酮、茄酮、苯甲醛、二氢猕猴桃内酯、亚麻酸甲酯、亚油酸甲酯、硬脂酸甲酯、2-乙酰基吡咯等,与对照相比新植二烯含量增加了54.6 μg/g;③经菌种发酵后,烟叶石油醚提取物含量没有显著性变化. ④感官实验表明,接种量为6.9%的评吸结果最好,这和香味成分的结果一致. 综上所述,西方许旺酵母对烟叶有一定的增香提质作用,接种量为6.9%时处理效果较好.

     

    Abstract: In order to improve aroma and quality of cured tobacco leaves, the aroma producing bacteria (Schwanniomyces occidentalis) screened from the surface of tobacco leaves were used the solid state fermentation of Xingyi C3F redryed tobacco leaves, and the chemical composition, aroma substances and contents of petroleum ether extract of tobacco leaves were determined. Finally, the tobacco leaves were fermented and rolled into cigarettes and sensory evaluation was carried out. The results showed that ① after fermentation, the ratio of sugar to alkali in the leaves increased by treated by inoculation amount of 6.9% and 13.8%, but there was no significant change in the ratio of potassium and chlorine. ② Fifty eight aromatic substances were detected in the leaves by treated by inoculation amount of 6.9%, and there were 50 flavor substances of them had the highest contents compared with other two groups, including benzyl alcohol, phenethyl alcohol, linalool, geranylgeraniol, solavetivone, phenylacetaldehyde, α-damascone, megastig-matrienone, nerylacetone, 3-hydroxy-beta-damascone, solanone, benzaldehyde, dihydroac-tinidiolide, methyl linolenate, methyl linoleate, methyl stearate, 2-acetylpyrrole. Compared with the control, the content of neophytadiene increased by 54.6 μg/g; ③after the fermentation, the petroleum ether extract of tobacco leaves was not significant changed. ④Sensory experiments showed that the best inoculation rate was 6.9%, which was consistent with the results of aroma components. To sum up, Schwanniomyces occidentalis has a certain effect of enhancement of aroma and quality on tobacco leaves, and the effect of inoculation amount of 6.9% was better.

     

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