Abstract:
In order to improve aroma and quality of cured tobacco leaves, the aroma producing bacteria (
Schwanniomyces occidentalis) screened from the surface of tobacco leaves were used the solid state fermentation of Xingyi C3F redryed tobacco leaves, and the chemical composition, aroma substances and contents of petroleum ether extract of tobacco leaves were determined. Finally, the tobacco leaves were fermented and rolled into cigarettes and sensory evaluation was carried out. The results showed that ① after fermentation, the ratio of sugar to alkali in the leaves increased by treated by inoculation amount of 6.9% and 13.8%, but there was no significant change in the ratio of potassium and chlorine. ② Fifty eight aromatic substances were detected in the leaves by treated by inoculation amount of 6.9%, and there were 50 flavor substances of them had the highest contents compared with other two groups, including benzyl alcohol, phenethyl alcohol, linalool, geranylgeraniol, solavetivone, phenylacetaldehyde, α-damascone, megastig-matrienone, nerylacetone, 3-hydroxy-beta-damascone, solanone, benzaldehyde, dihydroac-tinidiolide, methyl linolenate, methyl linoleate, methyl stearate, 2-acetylpyrrole. Compared with the control, the content of neophytadiene increased by 54.6 μg/g; ③after the fermentation, the petroleum ether extract of tobacco leaves was not significant changed. ④Sensory experiments showed that the best inoculation rate was 6.9%, which was consistent with the results of aroma components. To sum up,
Schwanniomyces occidentalis has a certain effect of enhancement of aroma and quality on tobacco leaves, and the effect of inoculation amount of 6.9% was better.