Abstract: For the aim of reveal the bioactivities and corresponding chemical constituents of Gentiana delavayi Franch, eight flavonoid glycosides were isolated from the n-butanol fraction of G. delavayi by various column chromatography involving silica gel, ODS, MCI and Sephadex LH−20. Their structures were identified based on NMR spectroscopic data analysis, together with comparison with previous data, to be luteolin−6−O−β−D-glucoside ( 1 ), vitexin ( 2 ), apigenin−7−O−β−D−glucopyranoside ( 3 ), isovitexin ( 4 ), puerarin ( 5 ), hypolaetin−8−O−β−D−glucoside ( 6 ), orientin ( 7 ) and isoorientin ( 8 ). Compounds 1 and 6 were isolated from this genus for the first time. In addition, antioxidant activity of the extracts from the plant, and compounds 1~8 were also tested. Compounds 6 and 7 showed strong DPPH free radical scavenging capacity, and compound 7 (9.74 ± 0.05 μg/mL) was the strongest one.