Abstract:
To determine a rapid and accurate method in pollen viability detection of
Nicotiana, in vitro germination was applied in testing the viability of the mature pollen stained with FDA-PI, TTC, I
2-KI and
Benzidine from
Nicotiana tabacum L 'K326',
N.
repanda and
N.
rustica. The results demonstrated that the most accurate and effective method was
in vitro germination combining TTC method, which could detect the pollen viability rapidly, while the other three staining methods were not applicable to the pollen viability detection of
Nicotiana.