Abstract:
In order to provide a technical support for seed production and large-scale application of eggplant cultivar ‘Yunqie No.3’ in Yunnan Province, SSR molecular marker technology was used to screen the specific primers for the parents of ‘Yunqie No.3’ (E26×E268), then the codominant polymorphic primers were screened in common eggplant cultivars in the market, and the final selected pairs of marker primers were applied. The purity of single plant of ‘Yunqie No.3’ was identified, and the results of plant purity test in the field were compared to verify the validity of SSR molecular marker for purity identification. The results showed that two pairs of primers (SM17 and SM29) were selected from 66 pairs of reported screening primers, which showed complementary and polymorphism band types in their parents. They could clearly distinguish ‘Yunqie No.3’ from the other six eggplant hybrids. The results of purity identification showed that detection of SSR molecular marker was the same as that of plant purity identification in the field, reaching 99%. The above results showed that SSR molecular markers were simple and efficient in detecting seed purity compared with the traditional field observation, and could be used as an assistant method to accelerate the breeding and promotion of eggplant varieties.