Abstract: Based on the method of water addition, the effects of water stress on nutrient accumulation and stoichiometric characters of Cymbopogon distans and Arundinella setosa were studied in Karst area of southern Yunnan. It was found that the content of elements of the two herbs were similar, among which the content of K, Ca, Mg and Na was relatively higher than other elements. In dry season, the accumulation of N, Ca, Al and Mn was affected by water addition, in particular, it significantly promoted the increase of N content. In wet season, N and K were enriched in A. setosa under water addition conditions. Moreover, compared with dry season, the content of K in the leaves of A. setosa decreased significantly in wet season. With the changes of soil moistures, the stability of macroelements in C. distans leaves was higher than that of A. setosa. The results of this study are important in guiding the management of plant ecosystems in degraded Karst basin under climate change.