Abstract:
The ferric uptake regulator (Fur) is an important regulation factor in bacteria. To explore the effect of
fur gene on iron uptake and biofilm formation ability of
Klebsiella pneumonia, and analyze the regulatory effect of Fur on the possible target gene
fyuA (receptor for
Yersinia), the unmarked
fur-deletion mutant and complementation strain C-Δ
fur were constructed through homologous recombination. Phenotype of wild strains, mutant strains and replenishment strains was determined by growth curve, CAS detection and crystal violet staining, and the transcription regulation mechanism of Fur on
fyuART-q was clarified by PCR and GFP fusion assay. The results showed that
fur affected the growth rate of bacteria; Deletion of the fur gene increased the production of siderophores and reduced the biofilm formation; The
fyuA gene was negatively regulated
by Fur under iron sufficient conditions.