Abstract: Malania oleifera Chun et S.Lee belongs to Olacaceae.Anovel protein,designated as malanin,was isolated and purified from the seeds of Malania oleifera by homogenization,extraction with phosphate solution,followed by ammonium sulfate precipitation and hydrophobic interaction chromatography.Malanin is a glycoprotein with a molecular weight of 61875 Du and an isoelectric point of 5.5,and it was linked with two bands of A-chain and B-chain by one or more disulfide bonds.The N-terminal amino acid sequences of malanin are DYPKLTFTTSin chain-A,and DETXTDEEFN(Xwas commonly C) in chain-B.The N-terminal sequences of A-chain and B-chain had been blasted by NCBI-BLASTPand other databases with the result of no significant similarity.MALDI-TOF-MS methods were applied to measure the peptide mass fingerprinting(PMF) of malanin-Aand malanin-B,but no significant protein similarity was found by the protein database of Peptldent,suggesting that malanin is a novel protein.