丙酮酸、乳酸对体外肌酸激酶活性的抑制作用

Effect of pyruvic acid or lactic acid on inactivation of creatine kinase

摘要: 应用酶活测定的方法分析了肌酸激酶在不同浓度的乳酸、丙酮酸中的活性变化.实验结果说明在失活过程中没有蛋白质的聚沉,失活与乳酸浓度、丙酮酸的浓度呈正相关.通过对比实验结果提示,丙酮酸离子、乳酸离子对肌酸激酶没有明显影响,丙酮酸、乳酸是通过其解离状态的H+改变了机体pH值而影响肌酸激酶活性的,作者就丙酮酸、乳酸对肌酸激酶影响及其意义进行探讨.

Abstract: The inactivation of creatine kinase in different lactic acid or pyruvic acid was determined by enzyme activity.The results shows that lactic acid or pyruvic acid cause inactivation of creatine kinase with no aggregation,and inactivation of creatine kinase caused by lactic acid or pyruvic acid is concentration-dependent.The acidification that results from ionization of lactic acid or pyruvic acid lowers the pH,and causes the inactivation of creatine kinase at last.The significance and effect of creatine kinase active changed by lactic acid or pyruvic acid have been expounded.