Abstract: Sixteen bacteria strains were isolated from Keke Salt Iake,Qinghai,China.Their amplification of 16S rDNA and sequencing were performed.The 16S rDNA PCR products of the tested strains were digested by two restriction enzymes (AfaⅠand HaeⅢ) and the results showed that 12 strains of them displayed great diversity with their restriction patterns.Phylogenetic analysis revealed that most of these strains belong to six genera of the family Bacillaceae:Halobacillus,Gracilibacillus,Amphibacillus,Virgibacillus,Bacillus and Salibacillus.In addition,from the phylogenetic analysis KN4 may be a new genus and KN2,KN3 and KN16 are probably three new species.The results showed that there are probably many new microorganisms in the extreme environments and Keke Salt Iake harbours a diverse of microbials.