王兵益, 孙静贤, 刘开庆, 杨小琴. RAPD-PCR稳定性的探索[J]. 云南大学学报(自然科学版), 2003, 25(4): 368-372.
引用本文: 王兵益, 孙静贤, 刘开庆, 杨小琴. RAPD-PCR稳定性的探索[J]. 云南大学学报(自然科学版), 2003, 25(4): 368-372.
shu
WANG Bing-yi, SUN Jin-xian, LIU Kai-qing, YANG Xiao-qin. The study on the stability of RAPD-PCR[J]. Journal of Yunnan University: Natural Sciences Edition, 2003, 25(4): 368-372.
Citation: WANG Bing-yi, SUN Jin-xian, LIU Kai-qing, YANG Xiao-qin. The study on the stability of RAPD-PCR[J]. Journal of Yunnan University: Natural Sciences Edition, 2003, 25(4): 368-372.
shu

RAPD-PCR稳定性的探索

The study on the stability of RAPD-PCR

    摘要
  • 摘要: 以滇牡丹(Paeonia delevayi)为实验材料,分别测试了Mg2+、模板DNA、聚合酶、引物等反应成分对产物的影响,以期探索RAPD-PCR体系中各成分对产物的影响,以及各成分之间相互作用的规律,为利用RAPD进行生物学研究的人们提供一些参考.

     

    Abstract: RAPD amplification of Paeonia delevayi was studied.In order to con struct a stable reaction system,the effect of Mg2+,dNTP,DNA templates,primers and DNA polymerase were tested respectively and this result may provide some reference to the use of this methods in biological stedies.

     

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