赖建华, 谭德勇, 钱伟, 何云刚, 余敏, 孙桂林. 合系35号水稻Q酶基因的克隆[J]. 云南大学学报(自然科学版), 2003, 25(2): 164-168.
引用本文: 赖建华, 谭德勇, 钱伟, 何云刚, 余敏, 孙桂林. 合系35号水稻Q酶基因的克隆[J]. 云南大学学报(自然科学版), 2003, 25(2): 164-168.
LAI Jian-hua, TAN De-yong, QIAN Wei, HE Yun-gang, YU Ming, SUN Giu-lin. The cloning of the He-35 rice’s Q enzyme gene[J]. Journal of Yunnan University: Natural Sciences Edition, 2003, 25(2): 164-168.
Citation: LAI Jian-hua, TAN De-yong, QIAN Wei, HE Yun-gang, YU Ming, SUN Giu-lin. The cloning of the He-35 rice’s Q enzyme gene[J]. Journal of Yunnan University: Natural Sciences Edition, 2003, 25(2): 164-168.

合系35号水稻Q酶基因的克隆

The cloning of the He-35 rice’s Q enzyme gene

  • 摘要: 支链淀粉含量是稻米品质的评价标准之一,控制支链淀粉合成的酶是淀粉分支酶.依据GenBank公布的日本水稻淀粉分支酶(Q酶)基因的cDNA序列合成相应引物,应用RT-PCR技术,SOE法成功地克隆到云南合系35号水稻Q酶基因的cDNA全长编码框2464bp.与日本水稻比较,合系35的Q酶基因有16个位点存在差异,并导致10个位点的氨基酸变化.

     

    Abstract: The content of starch-branching is one of the quality estimation standard in rice,the starch branching synthesize is controlled by the starch branching enzyme.According to the cDNA sequence of starch branching enzyme (Q enzyme) gene from Japanese rice that was published in Genbank, we designed and synthesized primers for amplifying this gene. Using the RT PCR and SOE method,we cloned successfully the full reading fram cDNA of the Q Enzyme gene in Yunnan He 35 rice,which contain 2?464?bp.Comparing with Q enzyme gene from Japanese rich, there are 16 base variation and 10 amino acid change in Yunnan He 35 rice.

     

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