Abstract:
The content of starch-branching is one of the quality estimation standard in rice,the starch branching synthesize is controlled by the starch branching enzyme.According to the cDNA sequence of starch branching enzyme (Q enzyme) gene from Japanese rice that was published in Genbank, we designed and synthesized primers for amplifying this gene. Using the RT PCR and SOE method,we cloned successfully the full reading fram cDNA of the Q Enzyme gene in Yunnan He 35 rice,which contain 2?464?bp.Comparing with Q enzyme gene from Japanese rich, there are 16 base variation and 10 amino acid change in Yunnan He 35 rice.