Abstract: It has been found that many components of collagens such as Collagen Ⅹ Ⅴ,Ⅹ Ⅷ NC domain can inhibit proliferation, migration of vessel endothelial cell as well as suppress tumor growth and metastased. Here a pair are designed if specific primers accordng to the uncleotide sequence of human collagen Ⅳ α1 chain NC1 domain which reported, then total RNA was extracted from human placenta tissue and human collagen Ⅳ α1 chain NC1 domain was amplified by RT-PCR. The result from nucleotide sequencing indicated that there is single nucleotide mutation from A to C at position 132 but deduced amino acid(Gly) is not changed. The human collagen Ⅳ α1 chain NC1 domain was then cloned into prodaryotic expression vector pPROEXHTb and overexpressed in E.coli BL21.